Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC 1-Propanesulfonic acid, 3-(bis(2-hydroxyethyl)amino)-2-hydroxy- | 68399-80-4 | MFCD00038353 | 98.0% | 100 G
DIPSO is a biological zwitterionic buffer with a useful pH range of 7.0 to 8.2. It can interfere with meiotic regulation in mouse oocytes and exhibits surfactant activity at 10 mM. This product is for research use only.
- Useful pH range of 7.0 to 8.2
- Can interfere with meiotic regulation in mouse oocytes
- Exhibits surfactant activity at 10 mM
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 24.5 Grams of Powder, Makes 5 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (5 liters, 24.5 grams of powder) is used for plant tissue culture applications that require precise control over pH levels and nutrient availability. The MES buffer stabilizes the pH environment, ensuring that plant cells receive consistent growth conditions. This medium is ideal for a variety of tissue culture applications, including shoot multiplication, regeneration, and plant propagation. The formulation includes essential vitamins and nutrients that support plant growth, making it an essential tool for researchers, plant breeders, and commercial growers. It is commonly used in agricultural laboratories and greenhouses to produce high-quality, healthy plant cultures.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.95% | 250 MG
MES monohydrate is an analytical standard intended for research and analytical applications. It is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments as a reference standard for assays.
- Used in cell culture
- Used in enzyme activity determination
- Used in electrophoresis
- Used in protein studies
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS
Bioworld ADA Buffer, 0.2M, pH 6.0, 250 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld BES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 100 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- It is a zwitterionic buffer and provides effective buffering capacity around pH 6.15.
- The pKa value changes with temperature, requiring consideration for its use at different temperatures.
- It shows good buffering properties in biological experiments.
- It exhibits poor affinity with metal ions, allowing its use in studies involving metal ions.
Medchemexpress LLC MOPS | 1132-61-2 | 100.0% | 1 ML
MOPS is a buffering agent utilized in biological applications. It effectively maintains the pH of mammalian cell culture media and significantly enhances the thermal stability of BSA (Bovine Serum Albumin).
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Enhances thermal stability of BSA.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 10 G
HEPPSO is a zwitterionic buffer. It has a working pH range of 7.1-8.5. HEPPSO demonstrates a relatively high capacity to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High capacity to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 100 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5
Bioworld BES, 1M, pH 7.0, 100 mL
BES bufferAlso known as N,N-Bis(hydroxyethyl)-2aminoethanesulfonic acid.Technical Information:pKA (25 C): 7.1Heavy Metals: <0.0005%. Appearance: LiquidBoiling Point : 404.34 °C (Predicted)Density : 1.44 g/cm3 (Predicted)Melting Point: 152-154 °CPH Value: 6.4-7.8
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255318.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
![eMolecules 7440-66-6 | Zinc, granular, ?10-+50 mesh, ACS | Oakwood Chemicals | MFCD00011291 | 65.380 | Zn | 99.000 | [Zn] | 1g | 480151265](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000224597.jpg-250.jpg)
eMolecules 7440-66-6 | Zinc, granular, ?10-+50 mesh, ACS | Oakwood Chemicals | MFCD00011291 | 65.380 | Zn | 99.000 | [Zn] | 1g | 480151265
Zinc, granular, ?10-+50 mesh, ACS | Oakwood Chemicals | 7440-66-6 | MFCD00011291 | 65.380 | Zn | 99.000 | [Zn] | 1g | 480151265
Bioworld ACES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
