Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
West Pharmaceutical Services Inc WEST PHARMACEUTICAL SERVICES INC
NC3936200 V-35 4432/50G SIL 1000/CS
Boston Bioproducts Inc HEPES Buffered Saline (2X) - 500ml
HEPES Buffered Saline (2X) - 500ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC 4-Morpholineethanesulfonic acid, sodium salt (2:1) | 117961-21-4 | 98.0% | 25 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Zwitterionic buffer, providing effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration for different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra, for molecular biology, 99.5 (T), 7365-45-9, MFCD00006158
HEPES BioUltra, for molecular biology, 99.5 (T)
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 100 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
ALADDIN SCIENTIFIC CORPORATION MES, 100g
useful pH range 5 5 - 6 7 pKa 6 1 MES is one of the Good s buffers the most extensively used biological buffers MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring It does not form complexes with majority of the metals used in environmental and biological studies It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes product description MES is one of the Good s buffers the most extensively used biological buffers MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring It does not form complexes with majority of the metals used in environmental and biological studies It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes
Boston Bioproducts Inc PIPES Buffer (1 M, pH 6.8) - 500ml
PIPES Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Hampton Research HR2-587/0.5 M MES monohydrate - 100 ml
HR2-587/0.5 M MES monohydrate - 100 ml. Please note items from this supplier are non-returnable.
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.
Dojindo Molecular Technologies Inc BES (100G), Good's buffer, CAS: 10191-18-1, pka=7.15, Active pH 6.6-8, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
eMolecules AstaTech / 14-DICHLOROANTHRAQUINONE / 0.25g / 475589680 / T71112 / 98.000 / 602-25-5 / MFCD00192002 / 277.100 / C14H6Cl2O2
AstaTech / 14-DICHLOROANTHRAQUINONE / 0.25g / 475589680 / T71112 / 98.000 / 602-25-5 / MFCD00192002 / 277.100 / C14H6Cl2O2
Dojindo Molecular Technologies Inc ADA (25G), Good's buffer, CAS: 26239-55-4, pka=6.6, Active pH 5.8-7.4, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 25 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier.
- Buffering agent with a useful pH range from 7.3 to 8.7.
- Reduces Aβ-aggregate-induced memory deficits.
- Rescues cognitive deficits in mice.
- Orally active.
- Penetrates the blood-brain barrier.